Skrining Lipid untuk Pengembangan Nanostructured Lipid Carrier (NLC) Mengandung Hesperetin
DOI:
https://doi.org/10.29313/bcsp.v6i2.26087Keywords:
Hesperetin, Nanostructured Lipid Carrier, Skrining lipidAbstract
Abstract. Hesperetin is a flavonoid compound with various pharmacological activities; however, its application is limited by its poor water solubility. One approach to overcome this limitation is the development of a Nanostructured Lipid Carrier (NLC)-based drug delivery system. This study aimed to perform lipid screening and formulate hesperetin into an NLC system, followed by the evaluation of its physicochemical characteristics. Lipid screening was conducted to determine the optimal combination of solid and liquid lipids with the best miscibility. Based on the screening results, glyceryl monostearate (GMS) was selected as the solid lipid and glyceryl stearate oil (oleum stearat) as the liquid lipid to form the lipid matrix. Subsequently, three NLC formulations with different lipid concentrations were prepared using Tween 80 as the surfactant through the hot homogenization method followed by sonication. The results demonstrated that the hesperetin-loaded NLC formulated with glyceryl monostearate and glyceryl stearate oil exhibited favorable physicochemical characteristics and showed potential as a drug delivery system to improve the stability and therapeutic effectiveness of hesperetin.
Abstrak, Hesperetin merupakan senyawa flavonoid yang memiliki berbagai aktivitas farmakologis, namun pemanfaatannya terbatas karena kelarutan air yang rendah. Salah satu upaya untuk mengatasi permasalahan tersebut adalah melalui pengembangan sistem penghantaran obat berbasis Nanostructured Lipid Carrier (NLC). Penelitian ini bertujuan untuk melakukan skrining lipid serta memformulasikan hesperetin dalam sistem NLC dan mengevaluasi karakteristik fisikokimianya. Skrining dilakukan untuk menentukan kombinasi lipid padat dan lipid cair yang memiliki ketercampuran terbaik. Berdasarkan hasil skrining, kombinasi gliseril monostearat (GMS) sebagai lipid padat dan oleum stearat sebagai lipid cair dipilih sebagai matriks lipid. Selanjutnya, dibuat tiga formula NLC dengan variasi konsentrasi lipid, menggunakan Tween 80 sebagai surfaktan melalui metode hot homogenization yang dilanjutkan dengan sonikasi.Berdasarkan hasil penelitian, formulasi NLC hesperetin dengan kombinasi GMS dan oleum stearat memiliki karakteristik fisikokimia yang baik dan berpotensi sebagai sistem penghantaran untuk meningkatkan stabilitas serta efektivitas hesperetin.
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