Optimasi Sintesis Nanopartikel Tembaga Menggunakan Ekstrak Etanol Daun Kelor sebagai Bioreduktor
DOI:
https://doi.org/10.29313/bcsp.v6i2.25685Keywords:
Nanopartikel tembaga, Moringa oleifera, Uv-Vis, PSA, Green synthesisAbstract
Abstract. Moringa leaves (Moringa oleifera) are a natural source of bioactive compounds such as flavonoids, polyphenols, and other secondary metabolites that have the potential to serve as bioreducing agents in the synthesis of copper nanoparticles (CuNPs). This study aims to investigate the potential of moringa leaf (Moringa oleifera) ethanol extract as a reducing agent in the green chemistry process for copper nanoparticles and to determine the physicochemical characteristics of the resulting copper nanoparticles (CuNPs). The synthesis of CuNPs was carried out by reacting the ethanol extract of Moringa leaves and the precursor solution CuSO4.5H2O at a ratio of 8:2 using a precursor concentration of 0.2M, then reacted at a temperature of 60°C for 3 hours. The formation of nanoparticles was observed through changes in the solution’s color and then analyzed using a UV-Vis spectrophotometer. Particle size characterization was performed using a particle size analyzer (PSA). The results of the study showed that ethanol extract of moringa leaves is capable of reducing Cu2+ ions into copper nanoparticles, as indicated by a color change and the formation of a UV-Vis absorption peak characteristic of nanoparticles at 651 nm, which indicates the formation of CuNPs. PSA analysis revealed a particle size of 153 nm with a PDI value of 0.2, indicating a relatively homogeneous particle size distribution. The FTIR results showed a shift in the C-H, C=O, C=C, and C-O indicating the involvement of bioactive compounds from the ethanol extract of moringa leaves in the reduction process of CuNPs.
Abstrak. Daun kelor (Moringa oleifera) merupakan salah satu bahan alam yang mengandung senyawa bioaktif seperti flavonoid, polifenol dan metabolit sekunder lainnya yang dapat berpotensi sebagai agen bioreduktor dalam sintesis nanopartikel tembaga (CuNPs). Penelitian ini bertujuan untuk melihat potensi ekstrak etanol daun kelor (Moringa oleifera) sebagai agen pereduksi dalam proses green chemistry nanopartikel tembaga serta menentukan karakteristik fisikokimia nanopartikel tembaga (CuNPs) yang dihasilkan. Sintesis CuNPs yang dilakukan dengan mereaksikan ekstrak etanol daun kelor dan larutan prekursor CuSO4.5H2O pada perbandingan 8:2 menggunakan konsenstrasi prekursor 0,2M, kemudian direaksikan pada suhu 60°C selama 3 jam. Pembentukan nanopartikel diamati melalui perubahan warna larutan lalu dianalisis menggunakan spektrofotometer UV-Vis. Karakterisasi ukuran partikel dilakukan menggunakan particle size analyzer (PSA). Hasil penelitian menunjukan bahwa ekstrak etanol daun kelor mampu mereduksi ion Cu2+ menjadi nanopartikel tembaga yang ditandai dengan perubahan warna, dan terbentuknya puncak serapan UV-Vis khas nanopartikel yaitu 651 nm yang mengindikasikan terbentuknya CuNPs. Analisis PSA menunjukan ukuran partikel 153 nm dengan nilai PDI 0,2 yang mengindikasikan distribusi partikel relatif homogen. Hasil FTIR menunjukan adanya perubahan pita serapan C-H, C=O, C=C, dan C-O yang mengindikasikan adanya keterlibatan senyawa bioaktif ekstrak etanol daun kelor dalam proses reduksi CuNPs.
References
Bezza, F. A., Tichapondwa, S. M., & Chirwa, E. M. N. (2020). Fabrication of monodispersed copper oxide nanoparticles with potential application as antimicrobial agents. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-73497-z
Crisan, M. C., Teodora, M., & Lucian, M. (2022). Copper nanoparticles: Synthesis and characterization, physiology, toxicity and antimicrobial applications. In Applied Sciences (Switzerland) (Vol. 12, Number 1). MDPI. https://doi.org/10.3390/app12010141
Das, P. E., Abu‐Yousef, I. A., Majdalawieh, A. F., Narasimhan, S., & Poltronieri, P. (2020). Green Synthesis of Encapsulated Copper Nanoparticles Using a Hydroalcoholic Extract of Moringa oleifera Leaves and Assessment of Their Antioxidant and Antimicrobial Activities. Molecules, 25(3). https://doi.org/10.3390/molecules25030555
Elisma, N., Emriadi, E., & Darmawi, A. (2023). Pengaruh Variasi Prekursor Terhadap Morfologi dan Aktivitas Antibakteri Nanopartikel Tembaga Menggunakan Reduktor Ekstrak Daun Gambir. REACTOR: Journal of Research on Chemistry and Engineering, 4(2), 68–75. https://doi.org/10.52759/reactor.v4i2.109
Hamdayani L.A, G. P. T. dan H. R. (2021). PENGARUH TINGKAT KEMATANGAN BUNNE (Antidesma bunius L.) SEBAGAI KOMPONEN PENGHASIL SENYAWA ANTIBAKTERI.
Kartini, K., Alviani, A., Anjarwati, D., Finna Fanany, A., Sukweenadhi, J., & Avanti, C. (2020). Process optimization for green synthesis of silver nanoparticles using indonesian medicinal plant extracts. Processes, 8(8). https://doi.org/10.3390/PR8080998
Perumalsamy, H., Balusamy, S. R., Sukweenadhi, J., Nag, S., MubarakAli, D., El-Agamy Farh, M., Vijay, H., & Rahimi, S. (2024). A comprehensive review on Moringa oleifera nanoparticles: importance of polyphenols in nanoparticle synthesis, nanoparticle efficacy and their applications. In Journal of Nanobiotechnology (Vol. 22, Number 1). BioMed Central Ltd. https://doi.org/10.1186/s12951-024-02332-8
Riska, A. M., & Putri, N. P. (2024). Green Synthesis TiO2 Menggunakan Ekstrak Daun Pepaya ( Carica Papaya L.) sebagai Bioreduktor yang Berpotensi dalam Aplikasi Fotokatalitik Green Synthesis of TiO2 Using Papaya (Carica papaya L.) Leaf Extract as a Bioreductor with Potential in Photocatalytic Applications. 9(1), 1–7.
Tanabe, I., Tanaka, Y. Y., Watari, K., Inami, W., Kawata, Y., & Ozaki, Y. (2020). Enhanced Surface Plasmon Resonance Wavelength Shifts by Molecular Electronic Absorption in Far- and Deep-Ultraviolet Regions. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-66949-z
