Validasi Metode KCKT Bahan Kimia Obat (BKO) pada Jamu Antidiabetes
DOI:
https://doi.org/10.29313/bcsp.v6i2.25358Keywords:
Bahan Kimia Obat, Glibenklamid, Jamu AntidiabetesAbstract
Abstract. Natural medicine is an Indonesian cultural heritage that has been used for generations as an alternative treatment for various diseases. One form of natural medicine that is widely consumed by the community is herbal medicine. This study aims to identify and determine the chemical content of glibenclamide in antidiabetic herbal medicine circulating in Bogor Regency using Thin Layer Chromatography and High Performance Liquid Chromatography methods, as well as to validate the High Performance Liquid Chromatography analysis method. A total of 8 antidiabetic herbal medicine samples were obtained from several herbal medicine shops in Bogor Regency. Qualitative analysis was carried out using Thin Layer Chromatography, while quantitative analysis was carried out using High Performance Liquid Chromatography. The results of Thin Layer Chromatography showed that sample G had Rf values of 0.44; 0.50; and 0.45 in three repetitions. The High Performance Liquid Chromatography method used has met all validation parameters, including linearity, accuracy, precision, selectivity, limit of detection, limit of quantitation, and system suitability, so it is declared valid for determining glibenclamide levels. Quantitative analysis results showed glibenclamide levels in sample G of 2,046%; 2,023%; and 2,072%, with an average concentration of 2,047%. Of the eight samples analyzed, one sample was identified as containing glibenclamide.
Abstrak. Obat bahan alam merupakan warisan budaya Indonesia yang telah digunakan secara turun-temurun sebagai alternatif pengobatan berbagai penyakit. Salah satu bentuk obat bahan alam yang banyak dikonsumsi masyarakat adalah jamu. Penelitian ini bertujuan untuk mengidentifikasi dan menentukan kadar bahan kimia obat glibenklamid dalam jamu antidiabetes yang beredar di Kabupaten Bogor menggunakan metode Kromatografi Lapis Tipis dan Kromatografi Cair Kinerja Tinggi, serta memvalidasi metode analisis Kromatografi Cair Kinerja Tinggi. Sebanyak 8 sampel jamu antidiabetes diperoleh dari beberapa toko jamu di Kabupaten Bogor. Analisis kualitatif dilakukan menggunakan Kromatografi Lapis Tipis, sedangkan analisis kuantitatif dilakukan menggunakan Kromatografi Cair Kinerja Tinggi. Hasil Kromatografi Lapis Tipis menunjukkan bahwa sampel G memiliki nilai Rf sebesar 0,44; 0,50; dan 0,45 pada tiga kali pengulangan. Metode Kromatografi Cair Kinerja Tinggi yang digunakan telah memenuhi seluruh parameter validasi, meliputi linearitas, akurasi, presisi, selektivitas, batas deteksi, batas kuantitasi, dan kesesuaian sistem, sehingga dinyatakan valid untuk penetapan kadar glibenklamid. Hasil analisis kuantitatif menunjukkan kadar glibenklamid pada sampel G sebesar 2,046%; 2,023%; dan 2,072%, dengan kadar rata-rata 2,047%. Dari 8 sampel yang dianalisis, 1 sampel teridentifikasi mengandung glibenklamid.
References
Badan Pengawas Obat dan Makanan Republik Indonesia. (2022). Laporan Tahunan Badan POM Tahun 2022. Program, 209–217. http://www.pom.go.id/ppid/2016/kelengkapan/laptah2015.pdf
ICH. (2023). Validation Of Analytical Procedures Q2(R2). 2(November).
Maggadani, B. P., Sugianto, N., & Hayun. (2020). Analytical method optimization and validation of glibenclamide and metformin hydrochloride in diabetic herbs product by thin-layer chromatography-densitometry. International Journal of Applied Pharmaceutics, 12, 83–87. https://doi.org/10.22159/ijap.2020.v12s1.FF014
Maspupah, T., Nina, N., Siagian, T. D., Pakhpahan, J., & Octavianie, G. (2022). Perilaku Pencegahan dan Risiko Kejadian Diabetes Melitus Tipe 2 pada Usia Produktif di Kabupaten Bogor Tahun 2021. Journal of Public Health Education, 2(1), 242–253. https://doi.org/10.53801/jphe.v2i1.66
Mir, K. B., Abrol, V., Wani, T. U., Jan, I., Singh, N., Khan, N. A., Dar, A. A., Sabri Sultan, R. M., Lone, S. A., Iesa, M. A. M., Alhag, S. K., Al-Shuraym, L. A., Helm, N., & AL-Farga, A. (2023). Validation and development of RP-KCKT method for quantification of glibenclamide in rat plasma and its application to pharmacokinetic studies in wistar rats. Heliyon, 9(11), e20876. https://doi.org/10.1016/j.heliyon.2023.e20876
Mulkin, A., Maarisit, W., Pareta, D., & Palandi, R. R. (2020). Identifikasi Bahan Kimia Obat (BKO) Glibenklamid pada Jamu Antidiabetes dengan Menggunakan Metode Kromatografi Lapis Tipis (KLT) dan Spektrofotodensitometri. The Tropical Journal of Biopharmaceutical, 2(2), 158–169.
Purnaningsih, N., Pramukanto, Q., & Sadiah, S. (2023). Program Pemanfaatan Lahan Kosong Fasilitas Umum untuk Taman Terapi Mandiri Diabetes Mellitus di Desa Benteng Kabupaten Bogor. 7, 111–120.
Sherma, J., & Rabel, F. (2019). Thin layer chromatography in the analysis of cannabis and its components and synthetic cannabinoids. Journal of Liquid Chromatography & Related Technologies, 42(19–20).
Utami, P. I., Firman, D., & Djalil, A. D. (2019). Identification of glibenclamide in antidiabetic jamu by high performace liquid chromatography method: Study in Purwokerto, Indonesia. Journal of Physics: Conference Series, 1402(5). https://doi.org/10.1088/1742-6596/1402/5/055065
Venita Syavera, & Muhamad Syazali. (2024). Peta Risiko Diabetes Melitus di Jawa Barat Tahun 2019-2023 dengan Pemodelan Spatio-Temporal. Sehat Rakyat: Jurnal Kesehatan Masyarakat, 3(4), 220–231. https://doi.org/10.54259/sehatrakyat.v3i4.3296
World Health Organization. (2016). Global Report on Diabetes. Isbn, 978, 88. https://doi.org/ISBN 978 92 4 156525 7
