Pengaruh Metode Ekstraksi terhadap Kadar Fenol dan Aktivitas Antioksidan Daun Cincau Hijau
DOI:
https://doi.org/10.29313/bcsp.v6i2.24579Keywords:
Daun Cincau Hijau, Maserasi, Ultrasonic-Assisted ExtractionAbstract
Abstract. This study aimed to compare the total phenolic content and antioxidant activity of 70% ethanol extracts of green grass jelly leaves obtained by maceration and Ultrasound-Assisted Extraction (UAE), and to determine the more effective extraction method. The results showed that the extraction yield obtained by maceration and UAE was 13.603% and 10.63%, respectively. The total phenolic content of the maceration and UAE extracts was 35.855 ± 3.08 mg GAE/g extract and 60.348 ± 6.177 mg GAE/g extract, respectively. The IC₅₀ values of antioxidant activity for the maceration and UAE extracts were 119.29 ± 11.384 mg/L and 82.117 ± 1.975 mg/L, respectively. Statistical analysis revealed significant differences in total phenolic content (p = 0.004) and antioxidant activity (p = 0.005) between the two extraction methods. These findings indicate that UAE is more effective than maceration in producing higher total phenolic content and stronger antioxidant activity in 70% ethanol extracts of green grass jelly leaves.
Abstrak. Penelitian ini bertujuan membandingkan kadar fenol total dan aktivitas antioksidan ekstrak etanol 70% daun cincau hijau hasil ekstraksi metode maserasi dan Ultrasound-Assisted Extraction (UAE), serta menentukan metode yang lebih efektif. Penelitian ini menunjukkan rendemen ekstrak maserasi sebesar 13,603% dan UAE sebesar 10,63%. Kadar fenol total ekstrak maserasi dan UAE berturut-turut adalah 35,855 ± 3,08 mg GAE/g ekstrak dan 60,348 ± 6,177 mg GAE/g ekstrak. Nilai IC₅₀ aktivitas antioksidan ekstrak maserasi sebesar 119,29 ± 11,384 mg/L, sedangkan ekstrak UAE sebesar 82,117 ± 1,975 mg/L. Analisis statistik menunjukkan perbedaan yang signifikan pada kadar fenol total (p = 0,004) dan aktivitas antioksidan (p = 0,005) antara kedua metode. Berdasarkan hasil tersebut, metode UAE lebih efektif dibandingkan maserasi dalam menghasilkan kadar fenol total dan aktivitas antioksidan yang lebih tinggi pada ekstrak etanol 70% daun cincau hijau.
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