Optimasi Isolasi Albumin Dari Belut (Monopterus Albus) Menggunakan Metode Heating Shock
DOI:
https://doi.org/10.29313/bcsp.v6i2.24540Keywords:
Albumin, Belut, HeatshockAbstract
Abstract. The swamp eel (Monopterus albus) is a potential source of albumin that can be developed as a raw material for albumin isolation. This study aimed to optimize the isolation of albumin from swamp eel using the heat-shock method with variations in temperature and heating time. Optimization was carried out using Response Surface Methodology (RSM) where temperature and time served as variables and albumin yield as the response variable. The results showed that temperature had a significant effect on albumin yield (p<0.05), whereas heating time and the interaction between temperature and time had no significant effect (p>0.05). The optimum condition for albumin isolation was predicted to be at a temperature of 80°C and a heating time of 75 minutes, resulting in an albumin yield 0,4546 %. SDS-PAGE analysis showed the presence of a protein band in the 60–72 kDa range, which was presumed to be albumin based on its molecular weight of approximately 66–67 kDa. Therefore, temperature was identified as the most influential factor affecting albumin isolation using the heat-shock method.
Abstrak. Belut (Monopterus albus) merupakan salah satu sumber albumin yang berpotensi dikembangkan sebagai bahan baku isolasi albumin. Penelitian ini bertujuan mengoptimasi isolasi albumin dari belut menggunakan metode heat shock dengan variasi suhu dan waktu pemanasan. Optimasi dilakukan menggunakan Response Surface Methodology (RSM) dengan suhu dan waktu sebagai variabel serta rendemen albumin sebagai respon. Hasil penelitian menunjukkan bahwa suhu berpengaruh signifikan terhadap rendemen albumin (p<0,05), sedangkan waktu dan interaksi suhu-waktu tidak berpengaruh signifikan (p>0,05). Kondisi optimum isolasi albumin dipediksi pada suhu 80°C dan waktu 75 menit dengan rendemen sebesar 0,4546 %. Hasil identifikasi menggunakan SDS-PAGE menunjukkan adanya pita protein pada kisaran 60–72 kDa yang diduga merupakan albumin sesuai dengan rentang berat molekul albumin sekitar 66–67 kDa. Dengan demikian, suhu merupakan faktor yang paling berpengaruh dalam proses isolasi albumin menggunakan metode heat shock.
References
Ashraf, M. A., Shen, B., Raza, M. A., Yang, Z., Amjad, M. N., Din, G. ud, Yue, L., Kousar, A., Kanwal, Q., & Hu, Y. (2025). Albumin: A Review of Market Trends, Purification Methods, and Biomedical Innovations. Current Issues in Molecular Biology, 47(5), 1–28. https://doi.org/10.3390/cimb47050303
Belinskaia, D. A., Voronina, P. A., Batalova, A. A., & Goncharov, N. V. (2021). Serum Albumin. 65–75.
Bernardi, M., Angeli, P., Claria, J., Moreau, R., Gines, P., Jalan, R., Caraceni, P., Fernandez, J., Gerbes, A. L., Brien, A. J. O., Trebicka, J., Thevenot, T., & Arroyo, V. (2020). Albumin in decompensated cirrhosis : new concepts and perspectives. 1127–1138. https://doi.org/10.1136/gutjnl-2019-318843
Borzova, V. A., Markossian, K. A., Chebotareva, N. A., & Kleymenov, S. Y. (2016). Kinetics of Thermal Denaturation and Aggregation of Bovine Serum Albumin. 1–29. https://doi.org/10.1371/journal.pone.0153495
Callum, J., Skubas, N. J., Bathla, A., Keshavarz, H., Clark, E. G., Rochwerg, B., Fergusson, D., Arbous, S., Bauer, S. R., China, L., Fung, M., Jug, R., Neill, M., Paine, C., Pavenski, K., Shah, P. S., Robinson, S., Shan, H., Szczepiorkowski, Z. M., … Wood, E. (2024). Use of Intravenous Albumin: A Guideline From the International Collaboration for Transfusion Medicine Guidelines. Chest, 166(2), 321–338. https://doi.org/10.1016/j.chest.2024.02.049
Dan, P., Parvalbumin, K., Teknologi, D., Perairan, H., & Perikanan, F. (2024). PATIN DAN GURAMI Roni Nugraha *, Novemi Gita Saftiri , Mala Nurilmala , Rizsa Mustika Pertiwi Purification and Characterization of Catfish and Gourami Parvalbumin. 27.
Fahrizal, A. (2025). Optimization of albumin extraction from carp ( Cyprinus carpio Linnaeus , 1758 ) under conditions of varying temperature and HCl solvent concentration. 5(1).
Fitriliyani, I., Suhanda, J., & Sari, D. K. (2019). Screening Profile Albumin dan Protein Jenis Ikan Konsumsi dari Perairan Umum Kalimantan Selatan. Prosiding Seminar Nasional Lingkungan Lahan Basah, 4(1), 18–22.
Khalid, W., Maggiolino, A., Kour, J., Arshad, M. S., Aslam, N., Afzal, M. F., Meghwar, P., Zafar, K., & Palo, P. De. (2023). Dynamic alterations in protein , sensory , chemical , and oxidative properties occurring in meat during thermal and non-thermal processing techniques : A comprehensive review. January, 1–19. https://doi.org/10.3389/fnut.2022.1057457
Li, H., Zhao, T., Li, H., & Yu, J. (2021). Effect of Heat Treatment on the Property , Structure , and Aggregation of Skim Milk Proteins. 8(September), 1–8. https://doi.org/10.3389/fnut.2021.714869
Masfar, V., Penetapan, A., Protein, K., & Ikan, E. (2023). JURNAL Kefarmasian Indonesia. 13(1), 50–58.
Paus, T., Hutapea, H., Madurani, K. A., Yudha, M., Nuril, M., Nina, A., & Kurniawan, F. (2023). Journal of Science : Advanced Materials and Devices Albumin : Source , preparation , determination , applications , and prospects. Journal of Science: Advanced Materials and Devices, 8(2), 100549. https://doi.org/10.1016/j.jsamd.2023.100549
Rabbani, G., & Ahn, S. N. (2021). Review: Roles of human serum albumin in prediction, diagnoses and treatment of COVID-19. International Journal of Biological Macromolecules, 193(PA), 948–955. https://doi.org/10.1016/j.ijbiomac.2021.10.095
Raoufinia, R., Mota, A., Keyhanvar, N., Safari, F., Shamekhi, S., & Abdolalizadeh, J. (2016). Overview of Albumin and Its Purification Methods. Tabriz University of Medical Sciences, 6(4), 495–507. https://doi.org/10.15171/apb.2016.063
Sumber, P., Alam, D., Penebaran, M., Benih, R., Endemik, I., Perairan, D. I., Daratan, U., Arsyita, M. A., Despa, D., Widyawati, R., & Purba, A. (2025). Seminar Nasional Keinsinyuran PERAN INSIYUR DALAM PROGRAM MAKAN BERGIZI GRATIS ( ANALISIS PROVINSI LAMPUNG TERHADAP PEMENUHAN KEBUTUHAN PROTEIN. 4(1).
Ukun MS Soedjanaatmadja, Nadiya Adnani, Wildan Abiyyi, Saadah Diana Rachman, Shabarni Gaffar, Safri Ishmayana, & Ari Hardiyanto. (2021). Albumin, as a Therapeutic Protein: Potential Source, Application, Isolation and Purification. International Journal of Research in Pharmaceutical Sciences, 12(3), 1922–1931. https://doi.org/10.26452/ijrps.v12i3.4794
Xie, Q. (2023). Effect of Body Weight on the Nutritional Content of Rice Field Eel , Monopterus albus. 2023. https://doi.org/10.1155/2023/9928273
Yuniati, R., Nurtari, R. Y., Annaafi, A. D., Priguna, T. M., Anggita, V. D., Kusumaningrum, N., Muslimin, I. S., Putra, F. E., Dermatologi, D., Kedokteran, F., & Diponegoro, U. (2024). ALBUMIN EKSTRAK IKAN GABUS The Effect of Long Heating and Aciditing Time on Albumin Levels in Snakehead Fish Extract. 27, 104–111.
