Pengujian Aktivitas Antioksidan dengan Menggunakan Metode DPPH dari Hidrolisat Protein Maggot BSF (Hermetia ilucens L.)
Abstract
Abstract. BSF maggot (Hermetia illucens L.) is an effective biodegradant in treating organic waste, producing high-value biomass rich in proteins known to have antioxidant activity. Oxidative stress due to the presence of free radicals is a common problem. This study aims to determine the antioxidant activity of Maggot BSF protein hydrolysate using DPPH method. The drying method used was freeze drying and oven, then the protein was hydrolyzed with alkalase, pepsin, and trypsin enzymes. The antioxidant activity testing method used DPPH method. The results of this study showed that antioxidant activity with IC50 values on protein hydrolysates of alkalase, pepsin, and trypsin enzymes were 111.83 ppm; 17.05 ppm; and 71.74 ppm, respectively.
Abstrak. Maggot BSF (Hermetia illucens L.) merupakan biodegradasi yang efektif dalam mengolah limbah organik, menghasilkan biomassa bernilai tinggi kaya akan protein yang diketahui memiliki aktivitas sebagai antioksidan. Stress oksidatif karena adanya radikal bebas merupakan masalah yang sering terjadi. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan hidrolisat protein Maggot BSF dengan metode DPPH. Metode pengeringan yang digunakan adalah freeze drying dan oven, kemudian protein dihidrolisis dengan enzim alkalase, pepsin, dan tripsin. Metode pengujian aktivitas antioksidan menggunakan metode DPPH. Hasil penelitian ini menunjukkan bahwa aktivitas antioksidan dengan nilai IC50 pada hidrolisat protein enzim alkalase, pepsin, dan tripsin secara berturut-turut sebesar 111,83 ppm; 17,05 ppm; dan 71,74 ppm.
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