Identifikasi Enzim pada Maggot Black Soldier Flies (H.illucens) yang Telah Dikeringkan
Abstract
Abstract. BSF maggot (Hermetia illucens) is the larva of the black soldier fly which is widely used as a waste bioconversion agent. This study aims to see the characterization and enzyme activity of amylase, protease, and lipase in dried BSF maggot. The determination of enzyme activity was carried out colorimetrically using a UV-Vis spectrophotometer. Identification of amylase enzyme was carried out using amylum and DNS reagent which was then measured absorbance at a wavelength of 540 nm. For protease enzyme, the test was carried out using casein and TCA (Trichloroacetic Acid) then, the absorbance was measured at a wavelength of 660 nm. In the lipase enzyme, the test was carried out using the Kwon and Rhee method and the absorbance was measured at a wavelength of 715 nm.The results of this study showed the presence of amylase enzyme activity in BSF maggot Freeze Drying (FD) of 0.0273 µg/ml and in BSF maggot oven (Ov) of 0.0298 µg/ml. The protease activity of BSF maggot FD and Ov was 0.2320 µg/ml & 0.3808 µg/ml, respectively. Then, the lipase enzyme activity produced in BSF FD and Ov maggot amounted to 0.5756 µg/ml and 0.5686 µg/ml. Based on the results of this study, it shows that the characterization test on BSF maggot (water content & ash content) meets the requirements and enzyme activity is not affected by the drying method.
Abstrak. Maggot BSF (Hermetia illucens) merupakan larva dari lalat tentara hitam yang banyak dimanfaatkan sebagai agen biokonversi limbah. Penelitian ini bertujuan untuk melihat karakterisasi dan aktivitas enzim amilase, protease, dan lipase pada maggot BSF yang telah dikeringkan. Penentuan aktivitas enzim dilakukan secara kolorimetri menggunakan spektrofotometer UV-Vis. Identifikasi enzim amilase dilakukan menggunakan amilum dan pereaksi DNS yang selanjutnya diukur absorbansi pada panjang gelombang 540 nm. Untuk enzim protease, pengujian dilakukan menggunakan kasein dan TCA (Trichloroacetic Acid) lalu, absorbansi diukur pada panjang gelombang 660 nm. Pada enzim lipase, pengujian dilakukan dengan menggunakan metode Kwon dan Rhee dan absorbansi diukur pada panjang gelombang 715 nm.Hasil dari penelitian ini menujukkan adanya Aktivitas enzim amilase pada maggot BSF Freeze Drying (FD) sebesar 0,0273 µg/ml dan pada maggot BSF oven (Ov) sebesar 0,0298 µg/ml. Pada aktivitas protease maggot BSF FD dan Ov masing-masing sebesar 0,2320 µg/ml & 0,3808 µg/ml. Kemudian, aktivitas enzim lipase yang dihasilkan pada maggot BSF FD dan Ov sebesar 0,5756µg/ml dan 0,5686 µg/ml. Berdasarkan hasil penelitian ini menunjukkan bahwa pengujian karakterisasi pada maggot BSF (kadar air & kadar abu) memenuhi syarat dan aktivitas enzim tidak dipengaruhi metode pengeringan.
References
BPOM. (2014). PERATURAN KEPALA BADAN PENGAWAS OBAT DAN MAKANAN REPUBLIK INDONESIA NOMOR 12 TAHUN 2014.
Cahyani, P. M., Maretha, D. E., & Asnilawati. (2020). Uji Kandungan Protein, Karbohidrat dan lemak pada Larva Maggot (Hermetia illucens) yang Diproduksi di Kalidoni Kota Palembang dan Sumbangsihnya pada Materi Insecta di Kelas X SMA/MA. Biolimi, 6(2), 120–128.
de Castro, M. D. L., & García, J. L. L. (2022). Analytical freeze-drying (pp. 11–41). https://doi.org/10.1016/S0167-9244(02)80004-X
Dewi, N. M. N. B. S. (2021). Analisa Limbah Rumah Tangga Terhadap Dampak Pencemaran Lingkungan. Jurnal Ganec Swara, 15(2), 1159–1164. http://journal.unmasmataram.ac.id/index.php/GARA
Kim, W., Bae, S., Park, K., Lee, S., Choi, Y., Han, S., & Koh, Y. (2011). Biochemical characterization of digestive enzymes in the black soldier fly, Hermetia illucens (Diptera: Stratiomyidae). Journal of Asia-Pacific Entomology, 14(1), 11–14. https://doi.org/10.1016/j.aspen.2010.11.003
Monisha, C., & Loganathan, M. (2022). Impact of drying methods on the physicochemical properties and nutritional composition of defatted black soldier fly ( Hermetia illucens ) pre‐pupae flour. Journal of Food Processing and Preservation, 46(1). https://doi.org/10.1111/jfpp.16184
Rachmawati, Buchori, D., Hidayat, P., Hem, S., & Fahmi, M. R. (2015). Perkembangan dan Kandungan Nutrisi Larva Hermetia illucens (Linnaeus) (Diptera: Stratiomyidae) pada Bungkil Kelapa Sawit. Jurnal Entomologi Indonesia, 7(1), 28. https://doi.org/10.5994/jei.7.1.28
Romano, N., Sinha, A., Powell, A., & Fischer, H. (2023). Mineral composition in black soldier fly (Hermetia illucens) larvae and resulting frass from fruit and their peels. Journal of Insects as Food and Feed, 9(1), 43–53. https://doi.org/10.3920/JIFF2022.0019
Salman, N., Nofiyanti, E., & Nurfadhilah, T. (2020). Pengaruh dan Efektivitas Maggot Sebagai Proses Alternatif Penguraian Sampah Organik Kota di Indonesia. Serambi Engineering, V(1).
Saucier, L., M’ballou, C., Ratti, C., Deschamps, M. H., Lebeuf, Y., & Vandenberg, G. W. (2020). Comparison of black soldier fly larvae pre-treatments and drying techniques on the microbial load and physico-chemical characteristics. Journal of Insects as Food and Feed, 8(1), 45–64. https://doi.org/10.3920/JIFF2021.0002
Seyedalmoosavi, M. M., Mielenz, M., Veldkamp, T., Daş, G., & Metges, C. C. (2022). Growth efficiency, intestinal biology, and nutrient utilization and requirements of black soldier fly (Hermetia illucens) larvae compared to monogastric livestock species: a review. In Journal of Animal Science and Biotechnology (Vol. 13, Issue 1). BioMed Central Ltd. https://doi.org/10.1186/s40104-022-00682-7
SIPSN. (2024). Sistem Pengelolaan Sampah Nasional. Https://Sipsn.Menlhk.Go.Id/Sipsn/.
Sumardi, S., Farisi, S., Ekowati, C. N., & Diana, M. S. (2019). Aktivitas dan Karakterisasi Enzim Protease Isolat Bacillus sp. (UJ132) Secara Kualitatif dan Kuantitatif. Jurnal Riset Akuakultur, 14(3), 193. https://doi.org/10.15578/jra.14.3.2019.193-199
Supriyatna, A., Amalia, D., Jauhari, A. A., & Holydaziah, D. (2015). Aktivitas Enzim Amilase, Lipase, dan Protease dari Larva Hermetia Illucens yang Diberi Pakan Jerami Padi. IX(2).
Yulianti, E., & Rakhmawati, A. (2017). PENGARUH SUHU DAN pH TERHADAP AKTIVITAS ENZIM FOSFATASE BAKTERI TERMOFILIK SUNGAI GENDOL PASCA ERUPSI MERAPI The INFLUENCE of TEMPERATURE and pH TOWARD the ACTIVITY of the PHOSPHATASE ENZYME FROMTHERMOPHILIC BACTERIA of GENDOL RIVER PASCA MERAPI ERUPTION. In Jurnal Prodi Biologi (Vol. 6).
