Nano-Alfamangostin : Gelasi Ionik dan Karakterisasi
Abstract
Abstract. Alpha-mangostin is a secondary metabolite isolated from mangosteen rind extract and is a xanthone derivative found in mangosteen rind (Garcinia mangostana L.) which has various pharmacological activities such as antioxidant, anticancer and anti-inflammatory. The use of alpha-mangostin as a therapeutic agent is limited due to its low solubility and limited bioavailability. This research aims to synthesize and characterize nano alpha-mangostin using the ionic gelation method. Synthesis was carried out by combining α-mangostin, chitosan, sodium tripolyphosphate (TPP), and alginate.
Nanoparticle characterization was carried out using Particle Size Analyzer (PSA), Transmission Electron Microscope (TEM), Fourier Transform Infrared (FTIR) spectroscopy, High-Performance Liquid Chromatography (HPLC), and zeta potential analysis. The results show that the nanoparticles in the study have an average size of 417,6 nm with a polydispersity index value of 1.704, which indicates quite good physical stability. Morphology using SEM and TEM interpreted the nanoparticles to be round in shape with a smooth surface, while FTIR analysis indicated successful interaction between α-mangostin and the polymer matrix. The percent content of encapsulated α-mangostin was 0.9%, and the zeta potential value of -31 mV indicated high electrostatic
Abstrak. Alfa-mangostin adalah metabolit sekunder yang diisolasi dari ekstrak kulit buah manggis dan merupakan turunan xanthone yang terdapat dalam kulit buah manggis (Garcinia mangostana L.) yang memiliki berbagai aktivitas farmakologi seperti antioksidan, antikanker, dan antiinflamasi. Penggunaan alfa-mangostin sebagai agen terapeutik terbatas karena kelarutannya yang rendah dan bioavaibilitasnya yang terbatas. Penelitian ini bertujuan untuk mensintesis dan mengkarakterisasi nano alfa-mangostin menggunakan metode gelasi ionik.
Sintesis dilakukan dengan menggabungkan α-mangostin, kitosan, natrium tripolifosfat (TPP), dan alginat. Karakterisasi nanopartikel dilakukan menggunakan Particle Size Analyzer (PSA), Transmission Electron Microscope (TEM), Fourier Transform Infrared (FTIR) spectroscopy, High-Performance Liquid Chromatography (HPLC), dan analisis zeta potensial. Hasil menunjukkan bahwa nanopartikel pada penelitian memiliki ukuran rata-rata 417,6 nm dengan nilai indeks polidispersitas 1,704 yang menunjukkan stabilitas fisik yang cukup baik. Morfologi menggunakan SEM dan TEM menginterpretasikan nanopartikel berbentuk bulat dengan permukaan halus, sedangkan analisis FTIR mengindikasikan keberhasilan interaksi antara α-mangostin dengan matriks polimer. Persen kadar α-mangostin yang terenkapsulasi adalah 0,9%, dan nilai zeta potensial -31 mV menunjukkan stabilitas elektrostatik yang tinggi.
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