Karakterisasi Enkapsulat Nanogel Kurkumin dengan Basis Alginat Dialdehid
DOI:
https://doi.org/10.29313/bcsp.v4i2.14447Keywords:
Alginat Dialdehid, Kurkumin, EnkapsulatAbstract
Abstract. Curcumin is a hydrophobic polyphenol that is insoluble in water but soluble in organic solvents. Curcumin has shortcomings such as low bioavailability, therefore it is necessary to do coating or encapsulation to increase stability. This research aims to characterize the nanoencapsulation of curcumin alginate dialdehyde based on the values of % transmittance, functional groups, particle size, zeta potential, and percent absorption. The nanogel results obtained were in samples 4 containing a ratio of alginate dialdehyde: sodium alginate (2:8) and 6 containing a ratio of alginate dialdehyde: sodium alginate (2:8) with a curcumin concentration of 1% because it produced a % transmittance of 94% in sample 4 and 92% in sample 6. Then in FTIR-ATR characterization, there are O-H and C=O groups while the results in sample 6 are aromatic C=C groups from these results indicate that there are alginate dialdehyde and curcumin groups in the nanogel sample. While the particle size characteristics in sample 4 are 277.244 nm ± 21.823 nm while in sample 6 are 204.833 nm ± 1.249 nm and the polydispersity index value in sample 4 is 0.299 ± 0.05 while in sample 6 is 1.175 ± 0.047. The result of zeta potential characterization in sample 4 is -2.867 mV ± 0.471 and in sample 6 is -3.767 mV ± 0.124. While the results of the percent of curcumin sorption obtained 17.605%.
Abstrak. Kurkumin merupakan polifenol hidrofobik yang tidak larut dalam air namun larut dalam pelarut organik. Kurkumin memiliki kekurangan seperti ketersediaan hayati yang rendah maka dari itu perlu dilakukan pelapisan atau enkapsulasi untuk meningkatkan stabilitas. Dalam penelitian yang dilakukan ini mempunyai tujuan untuk mengkarakterisasi nanoenkapsulasi kurkumin alginat dialdehid berdasarkan nilai % transmitan, gugus fungsi, ukuran partikel, zeta potensial, dan persen penjerapan. Hasil nanogel yang diperoleh yaitu pada sampel 4 yang mengandung perbandingan alginat dialdehid : natrium alginat (2:8) dan 6 yang mengandung perbandingan alginat dialdehid : natrium alginat (2:8) dengan konsentrasi kurkumin 1% karena menghasilkan % transmitan 94% pada sampel 4 dan 92% pada sampel 6. Kemudian pada karakterisisasi FTIR-ATR yaitu terdapat gugus O-H dan C=O sedangkan hasil pada sampel 6 yaitu terdapat gugus C=C aromatik dari hasil tersebut menandakan bahwa terdapatnya gugus alginat dialdehid dan kurkumin dalam sampel nanogel. Sedangkan pada karakteristik ukuran partikel pada sampel 4 yaitu 277,244 nm ± 21,823 nm sedangkan pada sampel 6 yaitu 204,833 nm ± 1,249 nm dan nilai indeks polidispersitas pada sampel 4 yaitu 0,299 ± 0,05 sedangkan pada sampel 6 yaitu 1,175 ± 0,047. Hasil karalterisasi zeta potensial yang pada sampel 4 yaitu -2,867 mV ± 0,471 dan pada sampel 6 yaitu -3,767 mV ± 0,124. Sedangkan hasil persen penjerapan kurkumin diperoleh 17,605%.
References
[2] Adassooriya, N., de Silva, P., & Amaratunga, G. (2015). A curcumin activated carboxymethyl cellulose-montmorillonite clay nanocomposite having enhanced curcumin release in aqueous media. Carbohydrate Polymers, 134, 695–699. https://doi.org/10.1016/j.carbpol.2015.08.030
[3] Almasi, H., Jahanbakhsh Oskouie, M., & Saleh, A. (2021). A review on techniques utilized for design of controlled release food active packaging. Critical Reviews in Food Science and Nutrition, 61(15), 2601–2621. https://doi.org/10.1080/10408398.2020.1783199
[4] Barros, J. A. R., Melo, L. D. R. de, Silva, R. A. R. da, Ferraz, M. P., Azeredo, J. C. V. de R., Pinheiro, V. M. de C., Colaço, B. J. A., Fernandes, M. H. R., Gomes, P. de S., & Monteiro, F. J. (2020). Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections. Nanomedicine: Nanotechnology, Biology, and Medicine, 24, 102145. https://doi.org/10.1016/j.nano.2019.102145
[5] Batool, S. R., Nazeer, M. A., Ekinci, D., Sahin, A., & Kizilel, S. (2020). Multifunctional alginate-based hydrogel with reversible crosslinking for controlled therapeutics delivery. International Journal of Biological Macromolecules, 150, 315–325. https://doi.org/10.1016/j.ijbiomac.2020.02.042
[6] Ceylan, Z., Meral, R., Kose, S., Sengor, G., Akinay, Y., Durmus, M., & Ucar, Y. (2020). Characterized nano-size curcumin and rosemary oil for the limitation microbial spoilage of rainbow trout fillets. Lwt, 134(April), 109965. https://doi.org/10.1016/j.lwt.2020.109965
[7] Chouhan, C., Rajput, R. P. S., Sahu, R., Verma, P., & Sahu, S. (2020). An Updated Review on Nanoparticle Based Approach for Nanogel Drug Delivery System. Journal of Drug Delivery and Therapeutics, 10(5-s), 254–266. https://doi.org/10.22270/jddt.v10i5-s.4465
[8] Eid, A. M. M., Elmarzugi, N. A., & El-Enshasy, H. A. (2013). Preparation and evaluation of olive oil nanoemulsion using sucrose monoester. International Journal of Pharmacy and Pharmaceutical Sciences, 5(SUPPL 3), 434–440.
[9] Grøndahl, L., Lawrie, G., Anitha, A., & Shejwalkar, A. (2019). Applications of alginate biopolymer in drug delivery. In Biointegration of Medical Implant Materials. Elsevier Ltd. https://doi.org/10.1016/B978-0-08-102680-9.00014-7
[10] Kohei Yabuuchi et al. (2023). Preparation of Cholesterol-Modified Hyaluronic Acid. Lee, W.-H., Loo, C.-Y., Bebawy, M., Luk, F., Mason, R., & Rohanizadeh, R. (2013). Curcumin and its Derivatives: Their Application in Neuropharmacology and Neuroscience in the 21st Century. Current Neuropharmacology, 11(4), 338–378. https://doi.org/10.2174/1570159x11311040002
[11] Lee, W. H., Loo, C. Y., Young, P. M., Traini, D., Mason, R. S., & Rohanizadeh, R. (2014). Recent advances in curcumin nanoformulation for cancer therapy. Expert Opinion on Drug Delivery, 11(8), 1183–1201. https://doi.org/10.1517/17425247.2014.916686
[12] Lestari, M. L. A. D., & Indrayanto, G. (2014). Curcumin. In Profiles of Drug Substances, Excipients and Related Methodology (Vol. 39). https://doi.org/10.1016/B978-0-12-800173-8.00003-9
[13] Liu, H. Y., Du, L., Zhao, Y. T., & Tian, W. Q. (2015). In vitro hemocompatibility and cytotoxicity evaluation of halloysite nanotubes for biomedical application. Journal of Nanomaterials, 2015. https://doi.org/10.1155/2015/685323
[14] Moga, A., Yandrapalli, N., Dimova, R., & Robinson, T. (2019). Optimization of the Inverted Emulsion Method for High-Yield Production of Biomimetic Giant Unilamellar Vesicles. ChemBioChem, 20(20), 2674–2682. https://doi.org/10.1002/cbic.201900529
[15] Muthal, A. D., Kshirsagar, S. J., Bhambere, D. S., Patil, M. P., Ahhirao, S. S., & Vishwakarma, R. P. (2022). a Brief Overview of Nanogel. Certified Journal │ Muthal et Al. World Journal of Pharmaceutical Research, 11(4), 1922–1942. https://doi.org/10.20959/wjpr20224-23678
[16] Pasaribu, S. P., Kaban, J., Ginting, M., & Sinaga, K. R. (2017). Synthesis of Dialdehyde Alginate by Oxidation Reaction Sodium Alginate with Sodium Metaperiodate. Jurnal Kimia Mulawarman, 14(2), 134–138.
[17] Pedrosa, S. S., Gonçalves, C., David, L., & Gama, M. (2014). A novel crosslinked hyaluronic acid nanogel for drug delivery. Macromolecular Bioscience, 14(11), 1556–1568. https://doi.org/10.1002/mabi.201400135
[18] Pradita, E. Y., & Wahyuni, S. (2023). Indonesian Journal of Chemical Science Nanogel Synthesis Of Chitosan-Alginate-Siam Orange ( Citrus nobilis Lour ) Extract and Its Antibacterial Activity. Indonesian Journal of Chemical Science, 12(1), 58–69.
[19] Rachmawati, H. (2013). Curcumin nanoforms promise better therapeutic values. International Journal of Research in Pharmaceutical Sciences, 4(2), 211–220.
[20] Rachmaniar, R., Permata Sari, I., Andareza, A., Fadillah, S., & Rizky Lestario, J. (2024). Karakteristik Nanoemulsi Isolat Brazilin dari Tanaman Kayu Secang (Caesalpinia sappan L.) Asli Indonesia. Majalah Farmasetika, 9(2), 205. https://doi.org/10.24198/mfarmasetika.v9i2.50495
[21] Sarker, B., Papageorgiou, D. G., Silva, R., Zehnder, T., Gul-E-Noor, F., Bertmer, M., Kaschta, J., Chrissafis, K., Detsch, R., & Boccaccini, A. R. (2014). Fabrication of alginate-gelatin crosslinked hydrogel microcapsules and evaluation of the microstructure and physico-chemical properties. Journal of Materials Chemistry B, 2(11), 1470–1482. https://doi.org/10.1039/c3tb21509a
[22] Singh, B., Kumar, A., & Rohit. (2020). Synthesis and characterization of alginate and sterculia gum based hydrogel for brain drug delivery applications. International Journal of Biological Macromolecules, 148, 248–257. https://doi.org/10.1016/j.ijbiomac.2020.01.147
[23] Shahbazizadeh, S., Naji-Tabasi, S., Shahidi-Noghabi, M., & Pourfarzad, A. (2021). Development of cress seed gum hydrogel and investigation of its potential application in the delivery of curcumin. Journal of the Science of Food and Agriculture, 101(15), 6505–6513. https://doi.org/10.1002/jsfa.11322
[24] Singh, N., Gill, V., & Gill, P. (2013). Nanogel Based Artificial Chaperone Technology : an Overview. American Journal of Advanced Drug Delivery, 1, 271–276.
[25] Soukoulis, C., & Bohn, T. (2018). A comprehensive overview on the micro- and nano-technological encapsulation advances for enhancing the chemical stability and bioavailability of carotenoids. Critical Reviews in Food Science and Nutrition, 58(1), 1–36. https://doi.org/10.1080/10408398.2014.971353
[26] Wahyu Ariani, L., & Wulandari. (2018). Formulasi Dan Stabilitas Fisik Sediaan Nanogel Minyak Biji Matahari. Repository.Stifar.Ac.Id, 1–9. https://repository.stifar.ac.id/Repository/article/download/229/293
[27] Yuan, J., Liu, R., Ma, Y., Zhang, Z., & Xie, Z. (2018). Curcumin Attenuates Airway Inflammation and Airway Remolding by Inhibiting NF-κB Signaling and COX-2 in Cigarette Smoke-Induced COPD Mice. Inflammation, 41(5), 1804–1814. https://doi.org/10.1007/s10753-018-0823-6
[28] Yuliani, S. H., Hartini, M., Pudyastuti, B., & Istyastono, E. P. (2016). Comparison of Physical Stability Properties of Pomegranate Seed Oil Nanoemulsion Dosage Forms With Long-Chain Triglyceride and Medium-Chain Triglyceride As the Oil Phase. Traditional Medicine Journal, 21(2), 93–98.
[29] Zarekar, N. S., Lingayat, V. J., & Pande, V. V. (2017). Nanogel as a Novel Platform for Smart Drug Delivery System. Nanoscience and Nanotechnology Research, Vol. 4, 2017, Pages 25-31, 4(1), 25–31. https://doi.org/10.12691/NNR-4-1-4
[30] Zeng, Q., Han, Y., Li, H., & Chang, J. (2014). Bioglass/alginate composite hydrogel beads as cell carriers for bone regeneration. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 102(1), 42–51. https://doi.org/10.1002/jbm.b.32978
