Uji Aktivitas Antibakteri Ekstrak Teh Putih (Camellia sinensis L.) terhadap Cutibacterium acnes
DOI:
https://doi.org/10.29313/bcsp.v4i2.13490Keywords:
Antibakteri, Jerawat, Ekstrak Teh PutihAbstract
Abstract. White tea (Camellia sinensis L.) is known to have high amounts of polyphenol content which has antibacterial activity. The aim of the research is to determine the antibacterial activity of white tea extract against acne-causing bacteria, Cutibacterium acnes. White tea extract was obtained by extraction with reflux method and ethanol 70%. Antibacterial activity test was conducted against C. acnes in concentrations of 0,025%, 0,05%, 0,1%, 0,2%, 0,4%, 0,8%, and 1,6% with agar well diffusion method. The results showed that MIC of white tea against C. acnes was 0,05% with inhibition zone 9,02 mm ± 1,01 and moderate antibacterial activity.
Abstrak. Teh putih (Camelilia sinensis L.) diketahui memiliki kandungan polifenol dalam jumlah tinggi yang diketahui bertanggung jawab terhadap aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak teh putih terhadap bakteri penyebab jerawat, yaitu Cutibacterium acnes. Ekstrak teh putih didapatkan dengan ekstraksi menggunakan metode refluks dan pelarut etanol 70%. Uji aktivitas antibakteri dilakukan terhadap C. acnes dalam konsentrasi 0,025%, 0,05%, 0,1%, 0,2%, 0,4%, 0,8%, dan 1,6% dengan metode difusi sumuran. Hasil penelitian menunjukkan bahwa ekstrak teh putih memiliki KHM terhadap C. acnes sebesar 0,05% dengan diameter zona hambat 9,02 mm ± 1,01 dan aktivitas antibakteri sedang.
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[24] Rahmanisa, S., & Oktaria, R. (2016). Pengaruh Epigallocatechin-3-Gallate (EGCG) pada Teh Hijau Terhadap Acne Vulgaris. Majority, 5(2), 101–105.
[25] Nurhayati, L. S., Yahdiyani, N., & Hidayatulloh, A. (2020). Perbandingan Pengujian Aktivitas Antibakteri Starter Yogurt dengan Metode Difusi Sumuran dan Metode Difusi Cakram. Jurnal Teknologi Hasil Peternakan, 1(2), 41. https://doi.org/10.24198/jthp.v1i2.27537
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[29] Winastri, N., Muliasari, H., & Hidayati, E. (2020). Aktivitas Antibakteri Air Perasan dan Rebusan Daun Calincing (Oxalis corniculata L.) terhadap Streptococcus mutans. Jurnal Ilmu-Ilmu Hayati, 19(2), 223–230.
[30] Syafriana, V., Purba, R. N., & Djuhariah, Y. S. (2021). Antibacterial Activity of Kecombrang Flower (Etlingera elatior (Jack) R.M. Sm) Extract against Staphylococcus epidermidis and Propionibacterium acnes. Journal of Tropical Biodiversity and Biotechnology, 6(1). https://doi.org/10.22146/jtbb.58528
[2] Fox, L., Csongradi, C., Aucamp, M., Du Plessis, J., & Gerber, M. (2016). Treatment Modalities for Acne. Molecules, 21(8). https://doi.org/10.3390/molecules21081063
[3] Durnova, N., Simakova, M., Isaev, D., Simakova, I., & Simakov, A. (2021). Morphology of Camellia Sinensis L. Leaves as Marker of White Tea Authenticity. Agronomy Research, 19(3), 1436–1445. https://doi.org/10.15159/AR.21.126
[4] Dias, Tomás, G., Teixeira, N. F., Alves, M. G., Oliveira, P. F., Silva, B. M., Maria Silva, B., & Fontes Oliveira, P. (2013). White Tea (Camellia sinensis (L.)): An-tioxidant Properties and Beneficial Health Effects. International Journal of Food Science, 2(2), 19–26. https://doi.org/10.19070/2326-3350-130005
[5] Ely, F. A., Taurhesia, S., & Indrawati, T. (2023). Antibacterial Effectiveness (Propionibacterium acnes) of Kombucha Face Toner Formula With Green Tea Leaf (Camellia sinensis L.). Medical Sains : Jurnal Ilmiah Kefarmasian, 8(3), 1101–1112.
[6] Yoda, Y., Hu, Z. Q., Zhao, W. H., & Shimamura, T. (2004). Different Susceptibilities of Staphylococcus and Gram Negative Rods to Epigallocatechin Gallate. Journal of Infection and Chemotherapy, 10(1), 55–58. https://doi.org/10.1007/s10156-003-0284-0
[7] Husni, E., Suharti, N., & Atma, A. P. T. (2018). Karakterisasi Simplisia dan Ekstrak Daun Pacar Kuku (Lawsonia inermis Linn) serta Penentuan Kadar Fenolat Total dan Uji Aktivitas Antioksidan. Jurnal Sains Farmasi & Klinis, 5(1), 12–16.
[8] Dipahayu, D., & Arifiyana, D. (2019). Pengaruh Metode Pengeringan Simplisia Daun Ubi Jalar Ungu (Ipomoea batatas (L.) Lamk) Varietas Antin 3 Terhadap Kadar Abu Ekstrak. Journal of Pharmacy and Science, 4(1), 11–14.
[9] Kementerian Kesehatan RI. (2017). Farmakope Herbal Indonesia (II). Kementerian Kesehatan Republik Indonesia.
[10] Maryam, F., Taebe, B., & Toding, D. P. (2020). Pengukuran Parameter Spesifik Dan Non Spesifik Ekstrak Etanol Daun Matoa (Pometia pinnata J.R & G.Forst). Jurnal Mandala Pharmacon Indonesia, 6(1), 1–12.
[11] Nurazizah, N. I., Darusman, F., & Aryani, R. (2020). Standarisasi Simplisia Daun Bidara Arab (Ziziphus spina-christi L.). Prosiding Farmasi, 6(2), 900–905. https://doi.org/10.29313/.v6i2.24072
[12] Marpaung, M., & Septiyani, A. (2020). Penentuan Parameter Spesifik Dan Nonspesifik Ekstrak Kental Etanol Batang Akar Kuning (Fibraurea chloroleuca Miers). Journal of Pharmacopolium, 3(2), 58–67.
[13] Narsa, A. C., Salman, A. A., & Prabowo, W. C. (2022). Identifikasi Metabolit Sekunder dan Profil Farmakognosi Kulit Bawang Merah (Allium cepa L) Sebagai Bahan Baku Farmasi Terbarukan. Jurnal Sains Dan Kesehatan, 4(6), 645–653. https://doi.org/10.25026/jsk.v4i6.1551
[14] Utami, Y. P., Halim Umar, A., Syahruni, R., & Kadullah, I. (2017). Standardisasi Simplisia dan Ekstrak Etanol Daun Leilem (Clerodendrum minahassae Teisjm. & Binn.). Journal of Pharmaceutical and Medicinal Sciences, 2(1), 32–39.
[15] Najihudin, A., Hindun, S., Rantika, N., Magfiroh, G., & Sujana, D. (2023). Karakterisasi Dan Studi Penapisan Fitokimia Daun Kelor (Moringa oleifera L.) Asal Garut Jawa Barat. Medical Sains : Jurnal Ilmiah Kefarmasian, 8(2), 679–686.
[16] Balakrishna, T., Vidyadhara, S., Sasidhar, R. L. C., Rucchita, B., & Prathyusha, E. V. (2016). A Review on Extraction Techniques. Indo American Journal of Pharmaceutical Sciences, 3(8), 880–891.
[17] Chua, L. S., Latiff, N. A., & Mohamad, M. (2016). Reflux Extraction and Cleanup Process by Column Chromatography for High Yield of Andrographolide Enriched Extract. Journal of Applied Research on Medicinal and Aromatic Plants, 3(2), 64–70. https://doi.org/10.1016/j.jarmap.2016.01.004
[18] Shi, L., Zhao, W., Yang, Z., Subbiah, V., & Suleria, H. A. R. (2022). Extraction and Characterization of Phenolic Compounds and Their Potential Antioxidant Activities. Environmental Science and Pollution Research, 29(54), 81112–81129. https://doi.org/10.1007/s11356-022-23337-6
[19] Utami, & Ulfa, A. (2023). Penetapan Kadar Flavonoid, Alkaloid Dan Fenolik Ekstrak Metanol Kulit Bawang Merah (Allium cepa L.) Menggunakan Metode Ekstraksi Sokletasi Dan Refluks. Jurnal Farmasi Malahayati, 6(1), 90–102.
[20] Senduk, T. W., Montolalu, L. A. D. Y., & Dotulong, V. (2020). Rendemen Ekstrak Air Rebusan Daun Tua Mangrove (Sonneratia alba). Jurnal Perikanan Dan Kelautan Tropis, 11(1), 9–15. https://ejournal.unsrat.ac.id/index.php/JPKT/index
[21] Saptowo, A., Supriningrum, R., & Supomo. (2022). Uji Aktivitas Antibakteri Ekstrak Kulit Batang Sekilang (Embeliaborneensis Scheff) Terhadap Bakteri Propionibacterium acnes dan Staphylococcus epidermidis. Al Ulum Sains Dan Teknologi, 7(2), 93–97.
[22] Sudarmi, K., Bagus, I., Darmayasa, G., & Muksin, K. (2017). Uji Fitokimia Dan Daya Hambat Ekstrak Daun Juwet (Syzygium cumini) Terhadap Pertumbuhan Escherichia coli dan Staphylococcus aureus ATCC. Jurnal Simbiosis, 5(2), 47–51. http://ojs.unud.ac.id/index.php/simbiosis
[23] Wu, M., & Brown, A. C. (2021). Applications of Catechins in the Treatment of Bacterial Infections. Pathogens, 10(5). https://doi.org/10.3390/pathogens10050546
[24] Rahmanisa, S., & Oktaria, R. (2016). Pengaruh Epigallocatechin-3-Gallate (EGCG) pada Teh Hijau Terhadap Acne Vulgaris. Majority, 5(2), 101–105.
[25] Nurhayati, L. S., Yahdiyani, N., & Hidayatulloh, A. (2020). Perbandingan Pengujian Aktivitas Antibakteri Starter Yogurt dengan Metode Difusi Sumuran dan Metode Difusi Cakram. Jurnal Teknologi Hasil Peternakan, 1(2), 41. https://doi.org/10.24198/jthp.v1i2.27537
[26] Sulistiyono, A., & Mutiara, E. (2023). Pengujian Bakteri Patogen pada Ikan Hias di Stasiun Karantina Ikan Pengendalian Mutu dan Keamanan Hasil Perikanan Palembang. Sriwijaya Bioscientia, 3(3), 1–9. https://doi.org/10.24233/sribios.3.3.2022.358
[27] Sari, R., Apridamayanti, P., & Pratiwi, L. (2022). Efektivitas SNEDDS Kombinasi Fraksi Etil Asetat Daun Cengkodok (Melasthoma malabathricum)-Antibiotik terhadap Bakteri Hasil Isolat dari Pasien Ulkus Diabetik. Pharmaceutical Journal of Indonesia, 7(2), 105–114. http://.pji.ub.ac.id
[28] Azizah, A. N., Ichwanuddin, & Marfu’ah, N. (2020). Aktivitas Antibakteri Ekstrak Etanol Teh Hijau (Camellia sinensis) Terhadap Pertumbuhan Staphylococcus epidermidis. Pharmasipha, 4(2), 15–23.
[29] Winastri, N., Muliasari, H., & Hidayati, E. (2020). Aktivitas Antibakteri Air Perasan dan Rebusan Daun Calincing (Oxalis corniculata L.) terhadap Streptococcus mutans. Jurnal Ilmu-Ilmu Hayati, 19(2), 223–230.
[30] Syafriana, V., Purba, R. N., & Djuhariah, Y. S. (2021). Antibacterial Activity of Kecombrang Flower (Etlingera elatior (Jack) R.M. Sm) Extract against Staphylococcus epidermidis and Propionibacterium acnes. Journal of Tropical Biodiversity and Biotechnology, 6(1). https://doi.org/10.22146/jtbb.58528
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2024-08-11
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